Determination of a novel anticonvulsant drug GIZh-290 in rat plasma by liquid chromatography–tandem mass spectrometry

GIZh-290 is a new derivative of 4-phenylpyrrolidone, based on the structures racetams that combine anticonvulsant and nootropic effects. The method high-performance liquid chromatography with mass spectrometric detection was developed validated for quantitative determination potential compound in rat blood plasma. For extraction analyte, liquid-liquid diethyl ether used. its selectivity, linearity, accuracy, precision, stability. linearity confirmed by high correlation coefficient 0.999. percent recovery from plasma 79.2 %. intra- inter-batch accuracy were 19.32 12.14 It found samples containing can be stored at ambient temperature during working day.